Figure 5
(a) Circular dichroism steady-state wavelength spectrum for Bm-YffB (0.05 mM) in NMR buffer collected at 298 K. (b) The CD thermal melt for Bm-YffB obtained by measuring the ellipticity at 216 nm in 2.0 K intervals between 283 and 353 K. (c) The first derivative of the thermal melt curve shows that the protein has a melting temperature of 326.6 K. |