The Protein Maker: an automated system for high-throughput parallel purification

Smith, E.R.; Begley, D.W.; Anderson, V.; Raymond, A.C.; Haffner, T.E.; Robinson, J.I.; Edwards, T.E.; Duncan, N.; Gerdts, C.J.; Mixon, M.B.; Nollert, P.; Staker, B.L.; Stewart, L.J.

doi:10.1107/S1744309111028776

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

IUCr IT WDC

home archive editors for authors for readers submit subscribe open access

view article

Figure 2
SDS–PAGE results for InvaB.07055.c (lanes 2–5) and InvaC.07055.b (lanes 7–10) during nickel-chelate chromatography purification on the Protein Maker. Lanes 1 and 6, molecular-weight markers (labeled on the left in kDa); lanes 2 and 7, pooled protein from Nickel 1; lanes 3 and 8, flowthrough of cleaved protein in buffer A from Nickel 2; lanes 4 and 9, buffer A wash from Nickel 2; lanes 5 and 10, removal of 6×His-Smt tag, 6×His-tagged protease and uncleaved protein with buffer B from Nickel 2.

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 67| Part 9| August 2011| Pages 1015-1021

doi:10.1107/S1744309111028776

Follow Acta Cryst. F

Search term		doi		Advanced search
Author		volume	page