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Figure 2
SDS–PAGE results for InvaB.07055.c (lanes 2–5) and InvaC.07055.b (lanes 7–­10) during nickel-chelate chromatography purification on the Protein Maker. Lanes 1 and 6, molecular-weight markers (labeled on the left in kDa); lanes 2 and 7, pooled protein from Nickel 1; lanes 3 and 8, flowthrough of cleaved protein in buffer A from Nickel 2; lanes 4 and 9, buffer A wash from Nickel 2; lanes 5 and 10, removal of 6×His-Smt tag, 6×His-tagged protease and uncleaved protein with buffer B from Nickel 2.

ISSN: 2053-230X
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