The Protein Maker: an automated system for high-throughput parallel purification

Smith, E.R.; Begley, D.W.; Anderson, V.; Raymond, A.C.; Haffner, T.E.; Robinson, J.I.; Edwards, T.E.; Duncan, N.; Gerdts, C.J.; Mixon, M.B.; Nollert, P.; Staker, B.L.; Stewart, L.J.

doi:10.1107/S1744309111028776

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

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Figure 3
SDS–PAGE analysis of 12 different cell-lysis buffer conditions described in Table 2

for CYP51A1. Lanes correspond to the load (L), wash (W) and elution (E) cycles conducted in parallel on the Protein Maker, with the same molecular-weight standards throughout (labeled on the left in kDa). Cell-lysis buffer scouting resulted in two conditions with optimal yields after affinity chromatography (red boxed bands).

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 67| Part 9| August 2011| Pages 1015-1021

doi:10.1107/S1744309111028776

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