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Figure 3
SDS–PAGE analysis of 12 different cell-lysis buffer conditions described in Table 2[link] for CYP51A1. Lanes correspond to the load (L), wash (W) and elution (E) cycles conducted in parallel on the Protein Maker, with the same molecular-weight standards throughout (labeled on the left in kDa). Cell-lysis buffer scouting resulted in two conditions with optimal yields after affinity chromatography (red boxed bands).

ISSN: 2053-230X
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