Figure 2
SDS–PAGE of BGLPf. Lane St, relative molecular-weight standards; lane 1, BGLPf prepared in reducing SDS–PAGE loading buffer (2% SDS and 710 mM β-mercaptoethanol) without heating; lanes 2, 3 and 4, BGLPf prepared in reducing SDS–PAGE loading buffer (2% SDS and 710 mM β-mercaptoethanol) and heated at 368 K for 5, 10 and 20 min, respectively. The molecular weight of monomeric BGLPf is 54.7 kDa. |