 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 2]](sw5049fig2mag.jpg)
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Figure 2
UV–Vis spectroscopy of PLP or PLP-bound (holo) BioA under various conditions. (a) Spectra of 200 µM holo BioA (purple), 200 µM PLP in 100 mM Tris pH 7.6 (black), 200 µM PLP in 100 mM HEPES pH 7.5 (red) and 400 µM PLP in water (cyan). Absorbance maxima at 390 and 420 nm correspond to free-aldehyde or internal-aldimine forms of PLP, respectively. (b) Spectra of different concentrations of holo BioA in storage buffer containing 1 mM DTT (yellow) or 0.1 mM TCEP (green). The peak near 345 nm (marked with an asterisk) is unidentified and occurs when holo BioA is in the presence of a thiol-containing reducing agent. |
![[Figure 2]](sw5049fig2.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
