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Figure 1
Characterization of the hydrodynamic properties of the ParE2–PaaA2 complex. (a) Analytical gel filtration shows that the ParE2–PaaA2 complex elutes as a single peak at 13.05 ml, corresponding to a molecular weight of 140 kDa and a hydrodynamic radius of 4.81 nm. The inset shows an SDS–PAGE (15% gel) analysis of the elution peak, which clearly demonstrates that both PaaA2 and ParE2 are present in the complex. (b) DLS measurements performed under the same conditions as the analytical gel-filtration experiment confirm that the ParE2–PaaA2 preparations contain a single particle with (within error limits) identical hydrodynamic properties.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 68| Part 6| May 2012| Pages 724-729
doi:10.1107/S1744309112015230
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