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Figure 1
Preparation of the extracellular region of Enpp1. (a) Construction of Enpp1. The domain organizations of Enpp1 and Enpp2 and a sequence alignment of the N-terminal regions of their SMB1 domains are shown. (b) Purification of Enpp1. Proteins were analyzed by SDS–PAGE and stained with SimplyBlue SafeStain. Lane 1, molecular-weight markers (labelled in kDa); lane 2, culture supernatant; lane 3, C-terminally tagged Enpp1 protein after P20.1-Sepharose chromatography; lane 4, purified Enpp1 protein after cleavage of the TARGET tag and gel-filtration chromatography.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 68| Part 7| June 2012| Pages 778-782
doi:10.1107/S1744309112019306
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