Expression, purification, crystallization and X-ray crystallographic studies of different redox states of the active site of thioredoxin 1 from the whiteleg shrimp Litopenaeus vannamei

Campos-Acevedo, A.A.; Garcia-Orozco, K.D.; Sotelo-Mundo, R.R.; Rudiño-Piñera, E.

doi:10.1107/S1744309113010622

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

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Figure 1
Micrograph of crystals of recombinant LvTrx obtained using the hanging-drop vapour-diffusion method. 10 mM DTT was added to every buffer used during all of the purification steps. 5 mM DTT was added to the crystallization droplet.

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 69| Part 5| April 2013| Pages 488-493

doi:10.1107/S1744309113010622

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