Serial femtosecond X-ray diffraction of 30S ribosomal subunit microcrystals in liquid suspension at ambient temperature using an X-ray free-electron laser

Demirci, H.; Sierra, R.G.; Laksmono, H.; Shoeman, R.L.; Botha, S.; Barends, T.R.M.; Nass, K.; Schlichting, I.; Doak, R.B.; Gati, C.; Williams, G.J.; Boutet, S.; Messerschmidt, M.; Jogl, G.; Dahlberg, A.E.; Gregory, S.T.; Bogan, M.J.

doi:10.1107/S174430911302099X

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

IUCr IT WDC

home archive editors for authors for readers submit subscribe open access

view article

Figure 1
Sample preparation (pre-filtration and injection) and experimental setup at the CXI endstation at the LCLS (Boutet & Williams, 2010

; Boutet et al., 2012

). The image of the gas dynamic virtual nozzle (GDVN) shows the liquid jet formed by the buffer containing ribosome microcrystals. The position of the LCLS beam interaction point is indicated by the red circle enclosed in the dashed rectangle. This is also shown by the white cross-hair in the real-time image.

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 69| Part 9| September 2013| Pages 1066-1069

https://doi.org/10.1107/S174430911302099X

Open

access

Follow Acta Cryst. F

Search IUCr Journals		doi		Advanced search
Author		volume	page