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Figure 1
(a) SDS–PAGE of RepSTK1 following digestion of RepSTK1 with pronase. Lane S, size markers (pertinent masses are given on the left in kDa); lane 1, RepSTK1 (no digestion); lanes 2, 3 and 4, RepSTK1 digested with 0.01, 0.1 and 1 µg pronase, respectively. R shows the position of the undigested protein, P shows the protease-resistant fragment. (b) Location of the Rep_trans motif with the RepSTK1 reading frame and extent of the truncated form RepSTK1(1–269) crystallized in this study. |