Identification, characterization and preliminary X-ray diffraction analysis of the rolling-circle replication initiator protein from plasmid pSTK1

Carr, S.B.; Mecia, L.B.; Phillips, S.E.V.; Thomas, C.D.

doi:10.1107/S1744309113023828

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

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Figure 3
Agarose gel showing the topoisomerase activity of Rep proteins. Lane 1, negatively supercoiled pCERoriSTK1; lanes 2, 3 and 4, plasmid after incubation with 64, 16 and 4 nM RepSTK1, respectively; lanes 5, 6 and 7, plasmid after incubation with 64, 16 and 4 nM RepSTK1_(1–269), respectively. SC, negatively supercoiled substrate DNA; OC, nicked open-circular intermediate; RC, relaxed covalently closed product.

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 69| Part 10| September 2013| Pages 1123-1126

doi:10.1107/S1744309113023828

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