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Figure 3
Agarose gel showing the topoisomerase activity of Rep proteins. Lane 1, negatively supercoiled pCERoriSTK1; lanes 2, 3 and 4, plasmid after incubation with 64, 16 and 4 nM RepSTK1, respectively; lanes 5, 6 and 7, plasmid after incubation with 64, 16 and 4 nM RepSTK1(1–269), respectively. SC, negatively supercoiled substrate DNA; OC, nicked open-circular intermediate; RC, relaxed covalently closed product. |