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Production, crystallization and X-ray of two nanobodies against the Duffy binding-like (DBL) domain DBL6∊-FCR3 of the Plasmodium falciparum VAR2CSA protein. Corrigendum
aStructural Biology Brussels, Department of Biotechnology (DBIT), Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussels, Belgium, bMolecular Recognition Unit (MoRe), Department of Structural Biology, VIB, Pleinlaan 2, B-1050 Brussels, Belgium, cInserm, UMRS665, F-75015 Paris, France, dUniversité Paris Diderot, F-75013 Paris, France, eInstitut National de la Transfusion Sanguine, F-75015 Paris, France, and fLaboratoire d'Excellence Gr-Ex, France
*Correspondence e-mail: reloris@vub.ac.be, stephanie.ramboarina@cea.fr
A correction is made to Fig. 1 and the text of the article by Vuchelen et al. (2013, Acta Cryst. F69, 270–274).
Keywords: pregnancy-associated malaria; VAR2CSA; DBL domain; nanobody; corrigendum.
In the article by Vuchelen et al. (2013![[Vuchelen, A., Pardon, E., Steyaert, J., Gamain, B., Loris, R., van Nuland, N. A. J. & Ramboarina, S. (2013). Acta Cryst. F69, 270-274.]](../../../../../../logos/arrows/f_arr.gif "Vuchelen, A., Pardon, E., Steyaert, J., Gamain, B., Loris, R., van Nuland, N. A. J. & Ramboarina, S. (2013). Acta Cryst. F69, 270-274.")
) there is a mistake in Fig. 1. The second line of Nb19 is a copy of the second line of Nb07, instead of the correct Nb19 line. This results in a difference of four amino acids. In §3, it is mentioned that there is one amino-acid substitution between the two nanobodies but this should be five (to include the four from the second line). Also the sequence identity should be 94% instead of 98%. A correct version of Fig. 1 is published here.
![[Figure 1]](bo9114fig1thm.gif) | Figure 1 Corrected sequence alignment of Nb2907 and Nb2919. The complementary determining regions (CDR) are shown in red boxes. |
References
Vuchelen, A., Pardon, E., Steyaert, J., Gamain, B., Loris, R., van Nuland, N. A. J. & Ramboarina, S. (2013). Acta Cryst. F69, 270–274. Web of Science CrossRef CAS IUCr Journals Google Scholar
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