Purification, crystallization and preliminary X-ray diffraction analysis of a novel keto-deoxy-d-galactarate (KDG) dehydratase from Agrobacterium tumefaciens

Taberman, H.; Andberg, M.; Parkkinen, T.; Richard, P.; Hakulinen, N.; Koivula, A.; Rouvinen, J.

doi:10.1107/S2053230X13031361

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

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Figure 2
At KDG dehydratase crystals (a) from the initial screening and (b) after optimizing the crystallization conditions. The optimized crystallization conditions consisted of 0.1 M Bicine pH 8.5, 0.2 M sodium formate, 15%(w/v) PEG MME 5000 and the crystals grew in 3–4 d.

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 70| Part 1| December 2014| Pages 49-52

doi:10.1107/S2053230X13031361

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