Expression, purification, crystallization and preliminary X-ray diffraction analysis of the novel modular DNA-binding protein BurrH in its apo form and in complex with its target DNA

Stella, S.; Molina, R.; Bertonatti, C.; Juillerrat, A.; Montoya, G.

doi:10.1107/S2053230X13033037

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

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Figure 1
BurrH expression and purification and protein–DNA complex formation. (a) SDS gel showing the purified BurrH protein. The left lane contains molecular-mass marker (labelled in kDa). (b) SEC–MALLS chromatogram. (c) Band-shift assay. The DNA target was incubated with and without BurrH protein for 1 h at 298 K. The mixture was separated on a native polyacrylamide gel. The arrow indicates the band corresponding to free DNA target.

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 70| Part 1| December 2014| Pages 87-91

doi:10.1107/S2053230X13033037

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