Expression, purification, crystallization and preliminary X-ray diffraction analysis of the novel modular DNA-binding protein BurrH in its apo form and in complex with its target DNA

Stella, S.; Molina, R.; Bertonatti, C.; Juillerrat, A.; Montoya, G.

doi:10.1107/S2053230X13033037

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

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Figure 2
BurrH crystallization. (a) Crystal of BurrH in the apo form. (b) Selenomethionine-substituted BurrH crystals in the apo form. (c) Crystals of native BurrH in complex with its target DNA. (d) SDS–PAGE gel confirming the crystallization of the BurrH–DNA complex stained with SimplyBlue Safe Stain to visualize the protein. (e) SDS–PAGE gel confirming the crystallization of the BurrH–DNA complex stained with SYBR-Gold to visualize the DNA. In both gels, lanes 1, 2 and 3 contain BurrH protein, the DNA target and the dissolved crystals, respectively.

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 70| Part 1| December 2014| Pages 87-91

doi:10.1107/S2053230X13033037

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