Expression, purification, crystallization and preliminary X-ray diffraction analysis of the novel modular DNA-binding protein BurrH in its apo form and in complex with its target DNA

Stella, S.; Molina, R.; Bertonatti, C.; Juillerrat, A.; Montoya, G.

doi:10.1107/S2053230X13033037

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

IUCr IT WDC

home archive editors for authors for readers submit subscribe open access

view article

Figure 3
Self-rotation function of the apo-BurrH crystals. SFCHECK (Vaguine et al., 1999

) and POINTLESS (Evans, 2006

) were used to check the data, indicating the absence of a translational Patterson peak and no twinning. We computed the self-rotation function of the apo-BurrH crystals using MOLREP (Vagin & Teplyakov, 2010

). Inspection of the stereographic projection detected strong peaks in the χ = 180° section and an absence of peaks in the χ = 120°, χ = 90° and χ = 60° sections. The peaks indicated the presence of twofold symmetry axes, which together with the Matthews coefficient data and the absence of substantial peaks in other sections suggested the presence of a dimer in the asymmetric unit.

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 70| Part 1| December 2014| Pages 87-91

doi:10.1107/S2053230X13033037

Follow Acta Cryst. F

Search term		doi		Advanced search
Author		volume	page