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Figure 4
The sitting-drop vapor-diffusion method is illustrated in this schematic diagram. The drop on the elevated platform, which is commonly 2–10 µl, consists of half stock protein solution and half reservoir solution which contains some concentration of a salt or polymer precipitant. About 0.5 ml of the reservoir solution is added to the bottom of the cell before sealing. By water equilibration through the vapor phase the drop ultimately approaches the reservoir in osmolarity, both raising the concentration of the precipitant in the drop and increasing the protein concentration there.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
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