view article
Figure 4
The sitting-drop vapor-diffusion method is illustrated in this schematic diagram. The drop on the elevated platform, which is commonly 2–10 µl, consists of half stock protein solution and half reservoir solution which contains some concentration of a salt or polymer precipitant. About 0.5 ml of the reservoir solution is added to the bottom of the cell before sealing. By water equilibration through the vapor phase the drop ultimately approaches the reservoir in osmolarity, both raising the concentration of the precipitant in the drop and increasing the protein concentration there.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 70| Part 1| December 2014| Pages 2-20
doi:10.1107/S2053230X13033141
Follow Acta Cryst. F
Sign up for e-alerts
E-alerts
Follow Acta Cryst. on Twitter
Twitter
Follow us on facebook
Facebook
Sign up for RSS feeds
RSS