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Figure 1
Structure of mouse p202 HINa bound to dsDNA. (a) Fluorescence polarization assays of the FAM-labelled dsDNA binding to mouse p202 HINa, mouse Aim2 HIN and human AIM2 HIN. The assays were performed in the presence of 15 nM 5′-FAM-labelled dsDNA and the indicated HIN proteins at various concentrations. (b) Graphical representations of the p202 HINa domain in complex with a 20 bp dsDNA in two views related by a 90° rotation around a vertical axis. Molecule A and molecule B of p202 HINa within the asymmetric unit are coloured blue and green, respectively, and chain C and chain D of dsDNA are shown in orange and yellow, respectively. In the left panel, the locations of the N-termini and C-termini of the two p202 HINa molecules are marked, and the dsDNA is shown as a surface model. In the right panel, molecule A is shown as surface representation coloured according to electrostatic potential (positive, blue; negative, red). (c) Ribbon representations of p202 HINa in two views related by a 60° rotation around a vertical axis. All β-strands are labelled in the left panel, and a structural comparison of two p202 HINa molecules with the human AIM2 HIN domain (coloured pink; PDB entry 3rn2 ) is shown on the right.

ISSN: 2053-230X
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