Figure 2
Superimposition of the WT and R80N mutant Mt-NDPK structures at the subunit interface. The intersubunit salt bridge Arg80–Asp93 found in WT Mt-NDPK (PDB entry 1k44
) was replaced by intersubunit hydrogen bonds in the R80N mutant involving the side-chain atoms of residues 93 and 96 and the main-chain carbonyl of Leu109 (PDB entry 4ane
). The C atoms of WT and R80N mutant Mt-NDPK are coloured orange and white, respectively. The 2Fo − Fc electron densities contoured at 2σ for chains A and C and water molecules of the R80N structure are coloured dark green, blue and red, respectively. The interface is marked with a broken line. |