 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 1]](no5036fig1mag.jpg)
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Figure 1
(a) Coomassie Brilliant Blue R-250-stained 12% SDS–PAGE gel of expressed Rv2779c. Lane M, molecular-weight markers (labelled in kDa); lane 1, total lysate after sonication; lane 2, flowthrough collected after loading; lane 3, sample washed with buffer A; lane 4, sample washed with buffer B; lane 5, sample washed with buffer C; lane 6, Ni2+–IDA-purified protein; lane 7, purified protein after size-exclusion chromatography. (b) Gel-filtration profile of Rv2779c from a Superdex S-200 HR10/300 column. The total column volume is 25 ml, while the void volume of the column is 8.43 ml. The protein eluted at 12.6 ml, consistent with an octameric association in solution. |
![[Figure 1]](no5036fig1.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
