Crystallization and preliminary X-ray analysis of a complex of the FOXO1 and Ets1 DNA-binding domains and DNA

Choy, W.W.; Datta, D.; Geiger, C.A.; Birrane, G.; Grant, M.A.

doi:10.1107/S2053230X13024795

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

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Figure 1
Protein and DNA constructs used in crystallization. (a) Human FOXO1-DBD protein region, residues 143–270. (b) Human Ets1-DBD protein region, residues 331–441. (c) The composite FOX:ETS enhancer sequence (De Val et al., 2008

) used in crystallization from the human ve-cadherin gene (CDH5) containing an ETS binding site (red) and a noncanonical forkhead binding site (blue). Complementary strands of the vecFOXETS DNA contain 19 paired bases and two overhanging bases on each of the 5′ ends.

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 70| Part 1| December 2014| Pages 44-48

doi:10.1107/S2053230X13024795

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