 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 2]](pg5024fig2mag.jpg)
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Figure 2
SDS–PAGE analysis of expressed and purified FOXO1-DBD protein; lane 1, molecular-weight marker proteins (Bio-Rad); lane 2, total E. coli cell lysate fraction after sonication; lane 3, soluble E. coli cell lysate fraction after centrifugation; lane 4, nickel-column elution fraction; lane 5, protein products following HRV3C protease cleavage; lane 6, column runthrough fraction from nickel-column reapplication post HRV3C cleavage; lane 7, pooled fractions of pure FOXO1-DBD protein following elution from size-exclusion medium and concentration. FOXO1-DBD is >95% pure and is homogeneous and appears to be a monomer in solution. |
![[Figure 2]](pg5024fig2.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
