view article

Figure 3
SDS–PAGE analysis of expressed and purified Ets1-DBD protein; lane 1, molecular-weight marker proteins (BioRad); lane 2, total E. coli cell lysate fraction after sonication; lane 3, soluble E. coli cell lysate fraction after centrifugation; lane 4, elution fraction from Fast Flow SP ion-exchange chromatography; lane 5, pooled fractions of pure Ets1-DBD protein following elution from size-exclusion medium and concentration. Ets1-DBD is >95% pure and is homogeneous and appears to be a monomer in solution.

ISSN: 2053-230X
Follow Acta Cryst. F
Sign up for e-alerts
Follow Acta Cryst. on Twitter
Follow us on facebook
Sign up for RSS feeds