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Figure 3
SDS–PAGE analysis of expressed and purified Ets1-DBD protein; lane 1, molecular-weight marker proteins (BioRad); lane 2, total E. coli cell lysate fraction after sonication; lane 3, soluble E. coli cell lysate fraction after centrifugation; lane 4, elution fraction from Fast Flow SP ion-exchange chromatography; lane 5, pooled fractions of pure Ets1-DBD protein following elution from size-exclusion medium and concentration. Ets1-DBD is >95% pure and is homogeneous and appears to be a monomer in solution.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 70| Part 1| December 2014| Pages 44-48
doi:10.1107/S2053230X13024795
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