Figure 4
Elution profile of the purified FOXO1-DBD (magenta) and Ets1-DBD (red) proteins, as well as the binary complex of Ets1-DBD–vecFOXETS DNA (dark blue) and a mixture of the binary complex Ets1-DBD–vecFOXETS DNA and the ternary complex of FOXO1-DBD–Ets1-DBD–vecFOXETS DNA (light blue) from an analytical size-exclusion column (Superdex 75 5/150 GL column; GE Healthcare). |