Figure 6
SDS–PAGE analysis of the ternary complex crystallization mixture and crystals; lane 1, fraction of pure Ets1-DBD protein following elution from size-exclusion medium and concentration; lane 2, fraction of pure FOXO1-DBD protein following elution from size-exclusion medium and concentration; lane 3, molecular-weight marker proteins (Bio-Rad); lane 4, the 1:1:1.3 molar ratio mixture of FOXO1-DBD, Ets1-DBD and vecFOXETS DNA used in crystallization experiments; lane 5, redissolved crystals of FOXO1-DBD, Ets1-DBD and vecFOXETS DNA. |