Figure 2
The final purified IF-BAR domain of human srGAP2 protein (residues 15–347). Protein samples were analyzed using 12% SDS–PAGE followed by Coomassie Blue staining. Lane 1, protein molecular-mass marker (labelled in kDa). Lane 2, the final purified protein after the gel-filtration chromatography step. The arrow indicates the band corresponding to the F-BAR domain of human srGAP2 protein (residues 15–347). |