Purification, crystallization and preliminary X-ray analysis of an HA17–HA70 (HA2–HA3) complex from Clostridium botulinum type C progenitor toxin

Iwasa, C.; Tonozuka, T.; Shinoda, M.; Sagane, Y.; Niwa, K.; Watanabe, T.; Yoshida, H.; Kamitori, S.; Takao, T.; Oguma, K.; Nishikawa, A.

doi:10.1107/S2053230X13032378

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

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Figure 1
SDS–PAGE of the complex of His-tagged HA17/C and MBP-tagged HA70/C. Lane M, molecular-mass marker (labelled in kDa); lane 1, crude extract; lane 2, amylose resin column eluate; lane 3, Ni–NTA agarose column eluate; arrow A, MBP-tagged HA70/C; arrow B, His-tagged HA17/C.

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 70| Part 1| December 2014| Pages 64-67

doi:10.1107/S2053230X13032378

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