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Figure 1
SDS–PAGE analysis of HsFH purification steps by nickel-affinity chromatography. Lane M, molecular markers (labelled in kDa). Lane 1, soluble fraction obtained after lysate centrifugation. Lane 2, washing step in the presence of buffer A containing 50 mM imidazole. Lane 3, HsFH enzyme (53.7 kDa) elution with buffer A containing 500 mM imidazole.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 70| Part 1| December 2014| Pages 120-122
doi:10.1107/S2053230X13033955
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