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Figure 5
(a, b) DSC scans of NaMNAT protein dissolved in different additive solutions. (a) NaMNAT at 0.324 mg ml−1 in buffer A (50 mM Tris–HCl pH 7.5, 100 mM NaCl, 10% glycerol, 1 mM DTT). B value = −9.6. (b) NaMNAT at 8.7 mg ml−1 in buffer B (50 mM Tris–HCl pH 7.5, 100 mM NaCl, 10% glycerol, 1 mM DTT, 50 mM arginine, 40 mM glutamic acid, 20 mM trehalose). B value = +1.6 × 10−4 mol ml g−2. (c) Crystals of NaMNAT prepared with PEG 8000 and malonate as precipitant agents from the solution in (b) containing 50 mM arginine, 40 mM glutamic acid and 20 mM trehalose. Polydispersity = 0.035. Crystallization B value = −5.1 × 10−4 mol ml g−2.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
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