 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 1]](rl5068fig1mag.jpg)
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Figure 1
Uba5 domain architecture and in vitro assay. (a) Summary of the Uba5 constructs used for activation assay. (b) Secondary-structure prediction of the C-terminal region of Uba5 using the PHD method. (c) In vitro activation of Ufm1 by Uba5. Different Uba5 recombinant proteins at 20 µM and Ufm1 at 100 µM were incubated in 100 µl reaction buffer consisting of 50 mM Tris–HCl pH 7.5, 150 mM NaCl, 5 mM MgCl2, 1 mM ATP at 303 K for different times. Reaction products at 10 µl were resolved by nonreducing 12% SDS–PAGE. The reaction time was 0 min for lanes 1, 5 and 9, 0.5 h for lanes 2, 6 and 10, 1 h for lanes 3, 7 and 11, and 2 h for lanes 4, 8 and 12. The arrows indicate the position of Uba5 fragments before reacting with Ufm1. Lane M contains molecular-mass markers (labelled in kDa). |
![[Figure 1]](rl5068fig1.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
