 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 1]](gx5225fig1mag.jpg)
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Figure 1
Unexpected plasmid caused by an irregular ligation. The method by which the substitutive mutations were introduced consists of two steps: (a) PCR with two primers whose sequences are not complementary is first performed; the self-ligation reaction of the long double-stranded DNA follows. Sequences of the primers and plasmids are shown in (b) and (c), respectively. The underlined sequence in (b) indicates the mutation site. Deletion of a base pair is shown in (c) as a hyphen. DNA and related amino-acid sequences are shown as one-letter codes. The stop codon is shown by an asterisk. Contrary to our expectation, a plasmid with a frame-shift mutation (M6ΔC) attributed to an irregular self-ligation process was found among the obtained clones. |
![[Figure 1]](gx5225fig1.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
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