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Figure 1
Pth1 purification and functional assay. (a) 12% SDS–PAGE gel. Lane MW, molecular-weight marker with sizes of the control proteins in kDa labeled. Lanes 1–6, Ni2+-affinity chromatography purification: lane 1, unbound fraction; lane 2, 25 mM imidazole wash; lanes 3–6, eluted fractions containing S. typhimurium Pth1; lane 7, total lysate; lane 8, soluble fraction of the lysate; lane 9, insoluble portion of the lysate; lane 10, S. typhimurium Pth1 after size-exclusion chromatography. (b) Functional assay for S. typhimurium Pth1 activity: bulk peptidyl-tRNA (lane 1) was cleaved by the purified recombinant enzyme (lane 2). The control band of 5S ribosomal rRNA in the samples is indicated.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 70| Part 7| July 2014| Pages 872-877
https://doi.org/10.1107/S2053230X14009893
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