Figure 4
Binding of carboplatin to the Nδ and/or N∊ atom of His15. (a) 65% MPD, 0.1 M citric acid buffer pH 4.0 crystal 1, (b) 65% MPD, 0.1 M citric acid buffer pH 4.0 crystal 2, (c) 0.2 M ammonium sulfate, 0.1 M sodium acetate, 25% PEG 4000 pH 4.6, (d) 0.1 M sodium citrate, 20% propanol, 20% PEG 4000 pH 5.6, (e) 2 M ammonium formate, 0.1 M HEPES pH 7.5. 2Fo − Fc maps (blue) are shown at the 1.2 r.m.s. contour level. Anomalous difference electron-density (orange) maps are shown at the 3.0σ contour level. The Pt atoms are shown in grey, C atoms are in green, O atoms are in red and N atoms are in blue. |