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Figure 1
15% SDS–PAGE analysis of purified APR1C stained with Coomassie Brilliant Blue. Lane 1, protein markers (labelled in kDa). Lane 2 corresponds to the product after thrombin protease cleavage. Lane 3 corresponds to the cleavage product obtained from GST-fused APR1C after elution with 10 mM GSH buffer. Lane 4 corresponds to the final concentrated APR1C showing high purity.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 70| Part 9| August 2014| Pages 1211-1214
doi:10.1107/S2053230X1401574X
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