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Figure 3
SDS–PAGE followed by Western blot analysis of Oxy and Fd complex crystals. (a) The dissolved crystals were verified by SDS–PAGE. Lane M, Precision Plus Protein Dual Color Standards (Bio-Rad; labelled in kDa); lane 1, Oxy solution used for crystallization; lane 2, Fd solution used for crystallization; lane 3, dissolved crystals of the complex. Approximately 3 µg protein was loaded per lane. (b) A Western blot stained with the anti-His antibody is shown; the lane numbers are the same as those in (a).

ISSN: 2053-230X
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