Crystallization and preliminary X-ray diffraction analyses of the redox-controlled complex of terminal oxygenase and ferredoxin components in the Rieske nonhaem iron oxygenase carbazole 1,9a-dioxygenase

Matsuzawa, J.; Aikawa, H.; Umeda, T.; Ashikawa, Y.; Suzuki-Minakuchi, C.; Kawano, Y.; Fujimoto, Z.; Okada, K.; Yamane, H.; Nojiri, H.

doi:10.1107/S2053230X14018779

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

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Figure 3
SDS–PAGE followed by Western blot analysis of Oxy and Fd complex crystals. (a) The dissolved crystals were verified by SDS–PAGE. Lane M, Precision Plus Protein Dual Color Standards (Bio-Rad; labelled in kDa); lane 1, Oxy solution used for crystallization; lane 2, Fd solution used for crystallization; lane 3, dissolved crystals of the complex. Approximately 3 µg protein was loaded per lane. (b) A Western blot stained with the anti-His antibody is shown; the lane numbers are the same as those in (a).

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 70| Part 10| September 2014| Pages 1406-1409

doi:10.1107/S2053230X14018779

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