Crystallization and preliminary X-ray diffraction analysis of the Sel1-like repeats of SEL1L

Jeong, H.; Lee, H.; Lee, C.

doi:10.1107/S2053230X14023115

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

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Figure 1
Protein purification. SDS–PAGE analysis showing the purification of recombinant SEL1L^trunc; lane 1, His-smt3-fused SEL1L^trunc after nickel–IMAC chromatography; lane 2, Ulp1 digestion of His-smt3-fused SEL1L^trunc; lane 3, SEL1L^trunc after the second nickel-IMAC step to remove His-smt3; lane 4, SEL1L^trunc after gel filtration. Lane M contains molecular-weight marker (AccuLadder Protein Size Marker, Bioneer; labelled in kDa).

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 70| Part 12| November 2014| Pages 1624-1627

doi:10.1107/S2053230X14023115

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