A simple technique to reduce evaporation of crystallization droplets by using plate lids with apertures for adding liquids

Zipper, L.E.; Aristide, X.; Bishop, D.P.; Joshi, I.; Kharzeev, J.; Patel, K.B.; Santiago, B.M.; Joshi, K.; Dorsinvil, K.; Sweet, R.M.; Soares, A.S.

doi:10.1107/S2053230X14025126

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

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Figure 3
Method for calculating the initial evaporation rate. We periodically measured the evaporation for droplets on covered and uncovered plates. The evaporation rate was calculated for droplets on the crystallization shelf of the uncovered plates and compared with the deduced evaporation rates for droplets on the crystallization shelf of plates covered with lids (using the technique described in §

2.1). These data were plotted as a function of the 6½ h measurement time for each of our tested designs. The initial rate of evaporation was obtained from the y intercept of the least-squares fit between a fifth-order polynomial and the evaporation-rate data. The data below were obtained from a 7.5 µl droplet on the crystallization shelf of a CrystalQuick X plate (this data point is boxed in Fig. 4

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 70| Part 12| November 2014| Pages 1707-1713

doi:10.1107/S2053230X14025126

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