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Figure 3
Method for calculating the initial evaporation rate. We periodically measured the evaporation for droplets on covered and uncovered plates. The evaporation rate was calculated for droplets on the crystallization shelf of the uncovered plates and compared with the deduced evaporation rates for droplets on the crystallization shelf of plates covered with lids (using the technique described in §[link]2.1). These data were plotted as a function of the 6½ h measurement time for each of our tested designs. The initial rate of evaporation was obtained from the y intercept of the least-squares fit between a fifth-order polynomial and the evaporation-rate data. The data below were obtained from a 7.5 µl droplet on the crystallization shelf of a CrystalQuick X plate (this data point is boxed in Fig. 4[link]).

ISSN: 2053-230X
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