A simple technique to reduce evaporation of crystallization droplets by using plate lids with apertures for adding liquids

Zipper, L.E.; Aristide, X.; Bishop, D.P.; Joshi, I.; Kharzeev, J.; Patel, K.B.; Santiago, B.M.; Joshi, K.; Dorsinvil, K.; Sweet, R.M.; Soares, A.S.

doi:10.1107/S2053230X14025126

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

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Figure 6
Smaller apertures reduce evaporation rate. We compared the measured evaporation rate for uncovered plates (orange) with the evaporation rate for plates that were covered with plate lids that had square apertures with 0.88 mm sides (left), 0.66 mm sides (middle) and 0.44 mm sides (right). The smaller apertures further reduced the deduced rates of evaporation (green) at the cost of requiring greater precision from the plate-preparation robot.

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 70| Part 12| November 2014| Pages 1707-1713

doi:10.1107/S2053230X14025126

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