Expression, crystallization and preliminary crystallographic study of the functional mutant (N60K) of nonstructural protein 9 from Human coronavirus HKU1

Chen, X.; Tan, Y.; Wang, F.; Wang, J.; Zhao, Q.; Li, S.; Fu, S.; Chen, C.; Yang, H.

doi:10.1107/S2053230X14023085

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

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Figure 1
Purification and crystallization of the N60K mutant of the nsp9 protein from HCoV-HKU1. (a) SDS–PAGE analysis of the purified N60K mutant of the nsp9 protein from HCoV-HKU1. The molecular masses of the marker are indicated in kDa. (b) Crystals of the N60K mutant of the nsp9 protein from HCoV-HKU1 grown by the hanging-drop method. A crystal with typical dimensions of 0.08 × 0.05 × 0.02 mm was used for subsequent diffraction and data collection.

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 70| Part 12| November 2014| Pages 1620-1623

doi:10.1107/S2053230X14023085

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