 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 2]](nj5204fig2mag.jpg)
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Figure 2
Radial distribution plots of the bacteriorhodopsin/purple membrane assembly (a), the intermediate state during the isolation of the protein from the membrane (b) and the assumed PDC of bacteriorhodopsin (c). (a) Radial distribution of bacteriorhodopsin/purple membrane assembly (BR) at a concentration of 20 mg ml−1 in 16 mM CHAPSO, 100 mM sodium chloride, 20 mM sodium acetate pH 5.0. (b) Radial distribution of BR at 20 mg ml−1 in 16 mM CHAPSO, 100 mM NaCl, 20 mM sodium acetate pH 5.0 after the addition of 2%(v/v) Triton X-100 in 20 mM sodium acetate as the first step of buffer exchange via a concentrator tube. The sample was reconcentrated for 30 min at 800g and then analyzed by in situ DLS. (c) The radial distribution of BR at 2.0 mg ml−1 after complete buffer exchange to 2%(v/v) Triton X-100 in 20 mM sodium acetate, centrifugation for 20 min and finally centrifugation at 16 000g in an Eppendorf tube prior to in situ DLS analysis. (d) Radial distribution of pure Triton-X micelles at a 2%(v/v) concentration in 20 mM sodium acetate as a control. The spot diameter represents the relative scattered light intensity of the detected particles in arbitrary units. |
![[Figure 2]](nj5204fig2.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
