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Figure 3
Radial distribution histograms characterizing the Mbp-dHBx fusion using in situ DLS analysis. (a) Mbp-dHBx at 2.0 mg ml−1 in 20 mM Tris–HCl, 50 mM NaCl, 1 mM DTT, 1 mM EDTA pH 7.4. (b) Mbp-dHBx (2.0 mg ml−1) in 20 mM Tris–HCl, 50 mM NaCl, 1 mM DTT, 1 mM EDTA pH 7.4, 6.3 mM TDM in the presence of the protease TEV. (c) Mbp-dHBx (2.0 mg ml−1) in 20 mM Tris–HCl, 50 mM NaCl, 1 mM DTT, 1 mM EDTA pH 7.4 and 9.8 mM TDM in the absence of TEV. (d) Control: pure 19 mM TDM.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
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