Figure 2
Fluorescence spectra in the context of the applied excitation spectra of the four aromatic amino acids tryptophan (a), tyrosine (b), phenylalanine (c) and histidine (d) in water at 1.8 mM. The dashed line indicates an excitation wavelength of 300 nm. For intrinsic fluorescence only tryptophan is relevant. Tyrosine fluorescence excitation is irrelevant for intrinsic fluorescence imaging owing to its weak absorption when illuminated with the applied illumination spectrum. |