 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 3]](en5560fig3mag.jpg)
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Figure 3
The diagrams on the left show the calculated excitation spectra at the sample for three different cover materials. On the right, intrinsic fluorescence images of the same glucose isomerase crystal covered with these three covering materials are shown. To allow a 1:1 comparison, the exposure time and all other camera parameters were kept identical for all three cases. Although the excitation spectra at short wavelengths (>300 nm) are vastly different owing to the absorption properties of the cover material, the intrinsic fluorescence image is still remarkably clear. The fact that even longer wavelengths are sufficiently effective to excite intrinsic fluorescence could be explained by tryptophan fluorescence properties, namely ineffective at wavelength larger than 300 nm but overcompensated by (a) the strong emission of the light source at these wavelengths and (b) the high permeability of the covering materials for this regime of the spectrum. |
![[Figure 3]](en5560fig3.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
