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Figure 5
(a, b) Hen egg-white lysozyme crystals among sodium chloride crystals illuminated with filtered radiation from a mercury vapor lamp (a) and white light (b). The protein crystals show the typical intrinsic blue fluorescence of tryptophan, while the salt crystals are invisible when excited with this spectrum. (c) Crystals of the 40-mer RNA L-oligonucleotide in complex with the protein L-CCP2 illuminated with bright light. (d) The same crystals when illuminated with the excitation spectrum; crystals of the protein–RNA complex crystals show the intrinsic blue fluorescence of tryptophan. (e) The same crystals after the addition of SYBR Gold fluorescence stain (∼1:1000); the crystals show characteristic green fluorescence, which would not be the case if nucleic acids were not present in the crystals.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
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