Figure 2
(a) Size-exclusion chromatography (SEC) of tCid1 (without GST fused or contaminating nucleic acids). A single symmetrical peak with a 260:280 nm ratio of 0.5 was observed when monitoring the elution at 280 and 260 nm. (b) SDS–PAGE analysis of the protein composition from SEC, demonstrating the presence of a single ∼45 kDa protein (tCid1). |