 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 1]](ft5061fig1mag.jpg)
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Figure 1
Purification of complexes of F1-ATPase and the ζ inhibitor protein from P. denitrificans. (a) Elution profile from a HiTrap Q column. Fractions of 5 ml were collected. The absorbance of the eluate was monitored at 280 nm (solid line) and the resistivity of the eluent was measured (dashed line). (b) Analysis of the protein compositions of peaks a–m in (a). The positions of subunits of the F1-ATPase and of the ζ inhibitor protein are indicated on the right. (c) Gel-filtration chromatography of the F1Δ∊–ζ complex from P. denitrificans [fractions j and k in (b)]. The absorbance of the eluate was monitored at 280 nm. The volume of each of fractions a–k (the bracketed region) was 0.5 ml. (d) Analysis by SDS–PAGE of fractions a–k in (c). The positions of subunits of the F1Δ∊–ζ complex are indicated on the right. |
![[Figure 1]](ft5061fig1.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
