view article
Figure 1
Overexpressed and purified rDer f 21 analyzed using 12% SDS–PAGE followed by Coomassie Blue staining. Lane 1, PageRuler Plus Prestained protein ladder (labelled in kDa; Thermo Scientific, USA). Lane 2, pellet of crude extract before IPTG induction as a negative control. Lanes 3–5, total protein, pellet and supernatant of crude extract after IPTG induction, respectively. Lane 6, purified rDer f 21 protein using Ni–NTA affinity chromatography.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 71| Part 11| November 2015| Pages 1396-1400
https://doi.org/10.1107/S2053230X1501818X
Follow Acta Cryst. F
Sign up for e-alerts
E-alerts
Follow Acta Cryst. on Twitter
Twitter
Follow us on facebook
Facebook
Sign up for RSS feeds
RSS