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Figure 2
Elution profile of PA4991 from analytical size-exclusion chromatography using a Superdex 200 10/300 column (GE Healthcare) equilibrated with 25 mM Tris–HCl buffer pH 8.0 containing 150 mM NaCl. The column was calibrated with ribonuclease A (13.7 kDa), chymotrypsinogen A (25 kDa), ovalbumin (43 kDa), albumin (67 kDa) and blue dextran (2 MDa). The calibration curve is shown in the inset.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 72| Part 2| January 2016| Pages 105-111
doi:10.1107/S2053230X15024437
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