view article

Figure 4
Thermofluor assay of protein melting temperature in the presence of stabilizing ligands. (a) Example melting curves for a protein of unknown function from Eubacterium siraeum (ZP_02421384.1) in the presence of various adenosine- and ribose-containing ligands (grey), adenosine diphosphate (ADP, green), adenosine triphosphate (ATP, red) and control sample with no ligand (blue dashed line). ADP and ATP result in a shift in melting temperature (ΔTm) of 3 and 8°C, respectively. (b) Matrix of 72 proteins of unknown function screened in a Thermofluor assay against a panel of 327 ligands. The ΔTm is indicated by the size and color of the data points ranging from 10 to 70°C. Data kindly provided by Anna Grezchnik of the Joint Center for Structural Genomics (JCSG).

ISSN: 2053-230X
Follow Acta Cryst. F
Sign up for e-alerts
Follow Acta Cryst. on Twitter
Follow us on facebook
Sign up for RSS feeds