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Figure 4
Thermofluor assay of protein melting temperature in the presence of stabilizing ligands. (a) Example melting curves for a protein of unknown function from Eubacterium siraeum (ZP_02421384.1) in the presence of various adenosine- and ribose-containing ligands (grey), adenosine diphosphate (ADP, green), adenosine triphosphate (ATP, red) and control sample with no ligand (blue dashed line). ADP and ATP result in a shift in melting temperature (ΔTm) of 3 and 8°C, respectively. (b) Matrix of 72 proteins of unknown function screened in a Thermofluor assay against a panel of 327 ligands. The ΔTm is indicated by the size and color of the data points ranging from 10 to 70°C. Data kindly provided by Anna Grezchnik of the Joint Center for Structural Genomics (JCSG).

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 72| Part 2| January 2016| Pages 72-95
doi:10.1107/S2053230X15024619
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