 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 1]](wd5272fig1mag.jpg)
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Figure 1
(a) SEC and multiangle light-scattering results for SP009239–491. Absorption at 280 nm is shown in blue. The molecular weights of the different oligomerization states are shown in red. (b) SEC elution profiles of monomeric and oligomeric SP009239–491 samples at different protein concentrations: monomer at 7, 21 and 54 µM in red, orange and yellow, and oligomer at 7, 21 and 54 µM in green, cyan and blue, respectively. SEC profiles of molecular-weight markers are shown in grey. SDS–PAGE of the elution fractions of oligomeric and monomeric samples at 21 µM are shown in cyan and orange boxes, respectively. (c) The proportion of oligomeric state from the pooled monomeric sample was calculated as the ratio of the integrals of peak1 (1.9–2.1 ml) and peak2 (2.2–2.4 ml). (d) The monomeric state proportion of the oligomeric sample was calculated as the ratio of the integrals of peak2 and peak1. |
![[Figure 1]](wd5272fig1.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
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